Journal:

Article Title: TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy

doi: 10.1038/sj.emboj.7601417

Figure Lengend Snippet: Requirement of TRPC3 and TRPC6 in Ang II-induced increases in membrane potential. (A) Western blots of the respective TRPC proteins. To identify the sizes of TRPC3 (C3), TRPC6 (C6), and TRPC7 (C7), each TRPC was overexpressed with recombinant adenoviruses. (B) Potentiating effects of TRPC3 and TRPC6 on changes in membrane potential by Ang II stimulation in LacZ-, TRPC3-, TRPC6-, and TRPC7-expressing cells. **P<0.01 versus Ang II stimulation of LacZ-expressing cells. NS means no significance from LacZ-expressing cells. (C–F) Effects of TRPC3 siRNAs (C, D) and TRPC6 siRNAs (E, F) on the expression of the respective TRPC proteins. (C, E) Representative Western blots with anti-TRPC3 (C) and anti-TRPC6 (E). (D, F) Effects of siRNAs of TRPC3 and TRPC6 on the average expression of native TRPC3, TRPC6, and TRPC7 proteins. (G) Effects of siRNAs of TRPC3 and TRPC6 on the maximal changes in DiBAC4(3) fluorescence intensity by Ang II (100 nM). Data are shown as the changes in membrane potentials (mV) calculated by in vivo calibration. **P<0.01 versus Ang II stimulation of control siRNA-treated cells (Control).

Article Snippet: Anti-TRPC6 and anti-TRPC3 antibodies were from Alomone.

Techniques: Western Blot, Recombinant, Expressing, Fluorescence, In Vivo

Journal:

Article Title: TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy

doi: 10.1038/sj.emboj.7601417

Figure Lengend Snippet: Requirement of TRPC3 and TRPC6 in Ang II-induced hypertrophic responses. Effects of siRNAs of TRPC3 and TRPC6 on Ang II-induced NFAT translocation (A, B), actin reorganization (C), and protein synthesis (D). Scale bar=20 μm. *P<0.05, **P<0.01 versus Ang II stimulation of control siRNA-treated cells (Control).

Article Snippet: Anti-TRPC6 and anti-TRPC3 antibodies were from Alomone.

Techniques: Translocation Assay

Journal:

Article Title: TRPC3 and TRPC6 are essential for angiotensin II-induced cardiac hypertrophy

doi: 10.1038/sj.emboj.7601417

Figure Lengend Snippet: Schema of Ang II-induced NFAT activation in cardiac myocytes. In cardiac myocytes, stimulation of AT1R induces NFAT activation through Gαq-PLC signaling pathway. DAG, generated by PLC activation, directly activates TRPC3 and TRPC6 (TRPC3/C6). Activation of TRPC3/C6 causes slow increases in the membrane potential to a positive direction (ΔΨ) and concomitantly increases the frequency of spontaneous firing due to activation of L-type Ca2+ channel. The Ca2+ influx though L-type Ca2+ channel activates calcineurin-NFAT pathway, which leads to hypertrophic responses in cardiomyocytes.

Article Snippet: Anti-TRPC6 and anti-TRPC3 antibodies were from Alomone.

Techniques: Activation Assay, Generated

Journal: Molecular medicine reports

Article Title: Growth inhibition effects of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on colorectal carcinoma cells and colon carcinoma-bearing mice.

doi: 10.3892/mmr.2016.4950

Figure Lengend Snippet: Figure 6. Effect of 5F treatment on the cell cycle, apoptosis and growth‑asso ciated proteins. Cells were treated with 20 µg/ml 5F for 24 h. (A) Western blots and (B) the relative density of p21, Bcl‑2, Bax, NF‑κB p65, IκB, survivin and β‑actin. **P<0.01 vs. the control. 5F, ent‑11α‑hydroxy‑15‑oxo ‑kaur‑16‑en‑19‑oic‑acid; p21, cyclin‑dependent kinase inhibitor 1A; Bcl‑2, B‑cell CLL/lymphoma 2; Bax, Bcl‑2‑associated X protein; NFκB, nuclear factor‑κ‑light‑chain enhancer of activated B cells; IκBα, nuclear factor of κ light polypeptide gene enhancer in B‑cells inhibitor, α.

Article Snippet: Immunoblots of 50 μg total protein were probed with primary antibodies overnight as follows: Rabbit anti-human polyclonal cyclin-dependent kinase inhibitor 1A (p21, Cip1) (p21; cat. no. BA0272; dilution, 1:200; Boster Bio-Engineering Ltd., Wuhan, China), rabbit anti-human polyclonal B-cell lymphoma 2 (Bcl-2; cat. no. ZS-492; dilution, 1:300; Zhongshan Golden Bridge Biotechnology, Co., Ltd., Beijing, China), mouse anti-mouse monoclonal Bcl-2-associated X protein (Bax; cat. no. ZS-7480; dilution, 1:300; Zhongshan Golden Bridge Biotechnology, Co., Ltd.), rabbit anti-human polyclonal nuclear factor κ-light-chain enhancer of activated B cells (NF-κB) p65 phosphorylated (p)-Ser536 (cat. no. 11014; dilution, 1:500; Signalway Antibody LLC., College Park, MD, USA), rabbit anti-human polyclonal nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor α (IκBα), rabbit anti-human polyclonal survivin (cat. no. sc-10811; dilution, 1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and β-actin (cat. no. sc-130656; dilution, 1:500; Santa Cruz Biotechnology, Inc.).

Techniques: Western Blot, Control